-dependent exocytosis from PC12 cells
نویسندگان
چکیده
Genes to Cells (2006) 11 , 659–672 Journal compilation © 2006 by the Molecular Biology Society of Japan/Blackwell Publishing Ltd. 659 DOI: 10.1111/j.1365-2443.2006.00970.x Blackwell Publishing Inc M lden, USA GTC enes to Cells 1365-2443 © Blackwell Publ shing Ltd ? 2006 1 6Original Artic e Ro of the ELKS/CAST family member in exocytosis E I ou et al. ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca 2+ -dependent exocytosis from PC12 cells
منابع مشابه
Modification of annexin II expression in PC12 cell lines does not affect Ca(2+)-dependent exocytosis.
The Ca2+/phospholipid/cytoskeletal-binding protein annexin II has been proposed to play an important role in Ca(2+)-dependent exocytosis; however, the evidence for this role is inconclusive. More direct evidence obtained by manipulating annexin II levels in cells is still required. We have attempted to do this by generating stably transfected PC12 cell lines expressing proteins which elevate or...
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Regulated secretion depends upon a highly coordinated series of protein-protein and protein-lipid interactions. Two phosphoinositides, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3-phosphate, are important for the ATP-dependent priming of the secretory apparatus prior to Ca(2+)-dependent exocytosis. Mechanisms that control phosphoinositide levels are likely to play an importa...
متن کاملSynaptotagmins I and IX function redundantly in regulated exocytosis but not endocytosis in PC12 cells.
Synaptotagmin I is considered to be a Ca2+ sensor for fast vesicle exocytosis. Because Ca2+-dependent vesicle exocytosis persists in synaptotagmin I mutants, there must be additional Ca2+ sensors. Multiple synaptotagmin isoforms co-reside on vesicles, which suggests that other isoforms complement synaptotagmin I function. We found that full downregulation of synaptotagmins I and IX, which co-re...
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Using PC12 cells that express transfected human growth hormone (hGH) as a secreted reporter protein, we have searched for Rab proteins that function in exocytosis. Among the Rab proteins tested, we found that besides the previously described Rab3 proteins, only members of the Rab11 family (Rab11a, 11b, and 25) impaired Ca2+-induced exocytosis. Rab11b, which is enriched in brain, had the stronge...
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